short note- simple and effective method for genomic dna extraction from plant and fungus for pcr-based analyses
نویسندگان
چکیده
0
منابع مشابه
A Simple and Rapid Leaf Genomic DNA Extraction Method for Polymerase Chain Reaction Analysis
In plants, secondary metabolites and polysaccharides interfere with genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The removal of such contaminants needs complicated and time-consuming protocols. In this study, a simple, rapid and efficient method for leaf DNA extraction was optimized. This method use small amount of plant mater...
متن کاملA RAPID AND SIMPLE METHOD OF DNA EXTRACTION FROM CLINICAL SPECIMENS CONTAINING MYCOBACTERIUM LEPRAE FOR PCR ASSAYS
We describe a rapid, simple, and reliable procedure for routine isolation of Mycobacterium leprae DNA from slit-skin swab specimens. This one-step DNA extraction method is based on the utilization of ChelexR 100, a chelating ion exchange resin. Slit-skin swab specimens from seven untreated leprosy patients at Baba Baghi Leprosy Hospital in Iran were processed by this procedure. The polymer...
متن کاملAn Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA
Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...
متن کاملA simple and rapid method for the preparation of plant genomic DNA for PCR analysis.
The polymerase chain reaction (PCR) has revolutionised the rapid analysis of mammalian genomic DNA (1). However, PCR is less useful in the analysis of plant DNA due to the difficulties in extracting nucleic acids from limited amounts of plant tissue. We have developed a method for the rapid extraction of small amounts of plant genomic DNA suitable for PCR analysis. The method is applicable to a...
متن کاملRapid DNA extraction from ferns for PCR-based analyses.
Pteridophytes are not as well characterized at the molecular and biochemical level as some higher plants. However, to aid conservation efforts (4), there is growing interest in understanding the population biology of many fern species that are under threat. DNAbased studies offer excellent tools for such work, but are hampered in ferns by difficulties in obtaining good-quality DNA from sufficie...
متن کاملExtraction of genomic DNA from yeasts for PCR-based applications.
We have developed a quick and low-cost genomic DNA extraction protocol from yeast cells for PCR-based applications. This method does not require any enzymes, hazardous chemicals, or extreme temperatures, and is especially powerful for simultaneous analysis of a large number of samples. DNA can be efficiently extracted from different yeast species (Kluyveromyces lactis, Hansenula polymorpha, Sch...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
چغندرقندجلد ۱۹، شماره ۲، صفحات ۱۷۵-۱۷۷
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023